Total Protein Quantification from Murine Teeth Using the Bead Ruptor 96 for Sample Preparation

Murine teeth are used as a model for study of topics relating to gene ontology, identification, and enamel analysis, allowing for downstream proteomic assays (Jágr, 2014). Prior to conducting specific assays relating to protein, the sample must be homogenized to lyse cells and release analytes of interest. Traditional lysis methods for tough sample types frequently involve mortar and pestle, liquid nitrogen, and enzymatic cocktails, which can be laborious inefficient procedures. 

The Bead Ruptor™ 96 bead mill homogenizer and the durable 2 mL Stainless Steel Lysing/Grinding Cryo tube provide scientists with an efficient front-end sample preparation solution to homogenize teeth samples, yielding homogenates that are suitable for downstream total protein quantification and setting the stage for in-depth protein analysis. 

Herein, we assess the Bead Ruptor 96 bead mill homogenizer and 2 mL Stainless Steel Lysing/Grinding tube in homogenization of murine teeth for incorporation into downstream BCA Total Protein assay.

Table 3: Calculated protein concentrations. Protein concentration was calculated from the equation generated from the standard curve.