Rapid Protein Extraction from Tissues with the Bead Ruptor 4 Mini Bead Mill Homogenizer

The Omni Bead Ruptor 4 (BR 4) bead mill homogenizer takes up a small footprint on the bench while still retaining the homogenizing power provided with Revvity’s bead ruptor family. The BR 4 is designed to efficiently lyse tissues for extraction of intracellular compounds, and is capable of homogenizing up to four 0.5 mL, 1.5 mL or 2 mL tubes or one 7 mL tube using bead media to assist in sample disruption. Screw cap or micro tubes containing bead media specific for the sample of interest are vigorously shaken to disrupt samples and release target analytes. Since the disruption occurs very quickly the molecular integrity of the target compound is maintained. This is particularly important for maintaining enzyme activity or for heat sensitive samples such as RNA.

Here, we demonstrate protein extraction from various tissue types using the Omni Bead Ruptor 4. Both protein extraction efficiency and diversity of proteins extracted are evaluated.

Table 1. Tissue sample mass and protein concentrations following homogenization on the Omni Bead Ruptor 4