Nucleic Acid Extraction from Rattus norvegicus Liver Using the Bead Ruptor Elite™ Bead Mill Homogenizer for Sample Preparation

Animal tissue is a widely used model for analysis of nucleic acids in downstream processes like PCR or next-generation sequencing (NGS) to allow for disease screening, gene identification and other relevant assays. Upstream of PCR or NGS, a nucleic acid extraction step is required which necessitates the input of lysed tissue cells. Traditional lysis methods involve enzymatic digestion, manual dissociation, or a combination of both, which can be time consuming and labor intensive processes for larger tissue samples. 

The Omni Bead Ruptor Elite™ bead mill homogenizer offers scientists a sample preparation workflow allowing for homogenization of large tissue samples in a quick and efficient process, yielding a homogenate conducive to downstream nucleic acid isolation, purification, and analysis. 

Herein, we evaluate the capability of the Omni Bead Ruptor Elite bead mill homogenizer in homogenization of Rattus norvegicus liver for downstream nucleic acid extraction and evaluation.

Figure 2, Table 3: Gel electropherogram, spectrophotometric data, and RIN value data obtained from tissue RNA eluate.