Nucleic Acid Extraction from Ocimum Basilicum using the Bead Ruptor Elite for Sample Preparation

Isolation and purification of nucleic acids from plant tissues is a required step before proceeding to downstream assays like PCR or genome sequencing. Areas of study that require nucleic acids as an input, include genome modification analysis in the case of genetically modified plants or pesticide resistance, and molecular identification of plant species. 

Sample preparation of plant tissues requires lysis to release analytes of interest from cells that comprise the plant tissue. Commonly used methods, including enzymatic digestion or mortar and pestle, can be time consuming and tedious processes. Implementing the Omni Bead Ruptor Elite™ bead mill homogenizer in front-end sample preparation is a robust solution to homogenize plant tissue. 

Herein, we evaluate the Omni Bead Ruptor Elite bead mill homogenizer as a sample preparation solution in front-end processing of plant tissue for downstream purification and quantification of nucleic acids.

Figure 1, Table 2: Gel electrophoresis and photometric assessment of DNA quantity and quality purified from two basil leaf samples.