Nucleic Acid Extraction from Liver Tissue: An Integrated and Automated Workflow Solution

Nucleic acid extraction from tissue commonly precedes downstream applications like quantitative PCR and Next Generation Sequencing, which are methods utilized to drive genomic-based research. The Omni Bead Ruptor Elite™ bead mill homogenizer and optimized 2 mL Bead Kits provide power and efficiency in sample homogenization releasing analytes of interest, while the chemagic™ 360 nucleic acid extractor automatically extracts genomic material from homogenized samples at low, medium and high throughput demands. 

Herein, we show integration of the Omni Bead Ruptor Elite bead mill homogenizer with the chemagic 360 nucleic acid extractor and JANUS® G3 automated workstation platforms by creating a workflow that incorporates sample preparation of tissue, custom-integrated liquid handling and automated nucleic acid extraction and provides the end user with nucleic acid suitable for further genomic analysis.

Figure 2: Liver DNA concentration from 12 samples. Lysate from samples 1-12 was prepared with Pro-K and RNase A. Average A260 /A280 from samples 1-12 was 1.90.