Nucleic Acid Extraction from HepG2 Cells: An Integrated and Automated Workflow Solution.

Applications featuring Next Generation Sequencing, quantitative PCR, or other genomics-based research using in-vitro cultured cells requires a nucleic acid extraction upstream of aforementioned methods. The Omni Bead Ruptor Elite™ bead mill homogenizer and 2 mL bead kits are a streamlined sample preparation solution that provides efficiency in cell lysis, releasing nucleic acid for extraction on the chemagic™ 360 automated nucleic acid extractor. 

Herein, we outline integration of the Omni Bead Ruptor Elite bead mill homogenizer with the chemagic™ 360 nucleic acid extractor and JANUS® G3 automated workstation, creating an end-to-end solution for sample preparation of in vitro cultured cells and nucleic acid extraction workflows.

Figure 2: HepG2 DNA concentration from 12 samples. Lysate from samples 1-12 was prepared with Pro-K and RNase A. Average A260 /A280 from samples 1-12 was 2.21.