High Throughput Lysis of E. coli for Multiple Analytes Using the Bead Ruptor 96

Bacterial research is an ongoing and ever-changing realm of science. In the past, researchers have been limited to testing bacteria in culture environments. Now, with newer technology, researchers rely less on older methods of plating or growing, and more on the latest genomic and proteomic methods for their research such as qPCR and mass spectrophotometry. To support these research needs, sample prep methods and products need to be developed so that bacteria can be processed for multiple types of downstream analysis. The 96-well plate format allows the researcher to conduct multiple types of analyses in one plate without the hassle of multiple samples in separate tubes. Here, we evaluated the lysis and recovery of DNA, RNA, and proteins from E. coli using the Omni Bead Ruptor™ 96 bead mill homogenizer and a 96-well plate with 0.1 mm ceramic bead media. All three analytes were evaluated for molecular integrity and concentration.

Figure 1: DNA quantities from duplicate samples.