High-Throughput Genomic DNA and Protein Extraction from Multiple Tissue Types using the Bead Ruptor 96 Bead Mill Homogenizer

There have been many studies of correlating gene and protein expression from single tissue samples 1,2,3,4. Clinical research samples can be especially challenging as sample sourcing and quantity are often limited. This is certainly the case for tissue samples of human origin for research use that represent rare disorders. To make the most of rare tissues, an ideal extraction method would be suitable for extracting a multitude of analytes, including nucleic acids, proteins, and small molecules. Furthermore, the method would be capable of processing multiple samples simultaneously.

The Omni Bead RuptorTM 96 bead mill homogenizer is capable of rapid tissue disruption via bead milling in 96 well plates, tubes or cryomilling in stainless steel chambers. Herein, we evaluate the Omni Bead Ruptor 96 bead mill homogenizer for high-throughput tissue disruption for the purification of genomic DNA and proteins. Genomic DNA integrity is evaluated, and the protein repertoire is analyzed via electrophoresis and bottom-up semi-quantitative proteomics.

Table 2: Average DNA and protein concentrations measured via spectrophotometry.