Traditional methods of tissue homogenization have limitations such as sample loss and low amounts of cells being available, which in turn yields a low amount of starting material that can make some experiments impossible. With such limitations from small amounts of starting material and difficult-to-process samples, consistency in data and consistency in experiments is more important than ever. High quality tissue homogenization and consistent shearing is pivotal to generate data.
Herein, we demonstrate a robust workflow for ChIP-Seq sample preparation and analysis from clinically relevant tissue types using the Omni Bead Ruptor Elite bead mill homogenizer to dissociate tissues and the PIXUL™ Multi-Sample Sonicator for ChIP-Seq sample preparation.
Figure 2: Overview of human tissues sonicated with PIXUL™ Multi- Sample Sonicator. All but one sample surpassed our internal QC metric of 70 % sonicated fragments. Kidney and adipose tissue nearly sonicate to 100 %. Some tissues, such as heart, are more challenging, but this sample also approached a 70 % sonication level which is considered acceptable for downstream ChIP-Seq analysis.