Drosophila melanogaster is a commonly used organism in many research studies. A reliable method for protein and nucleic acid extraction is a critical step toward understanding the molecular processes that occur in Drosophila. This study demonstrates a fast and efficient method of extracting proteins and DNA from Drosophila using the Bead Ruptor Elite™ bead mill homogenizer.
Figure 1: Drosophila protein extracts analyzed by SDS PAGE. Lane 1: Protein ladder. Lane 2-3: 30 mg of Drosophila homogenate. Lane 4-5: 50 mg of Drosophila homogenate. Lane 6: Protein ladder