Recent advances in bead milling technology, including the Omni Bead Ruptor Elite™ bead mill homogenizer has enabled the rapid and efficient lysis of plant material which is achieved through vertical intra-tube bead motion inducing high impact forces to rapidly dissociate samples. The Omni Bead Ruptor Elite bead mill homogenizer supports bead mill based homogenization of samples in volumes ranging from 0.5 mL to 50 mL. For larger sample volumes up to 50 mL, bead milling in the Omni Bead Ruptor Elite is achieved through a spiral grinding motion as the 50 mL tube is positioned horizontally. The study herein seeks to determine if intra-tube bead movement effects sample disruption and protein recovery from plant samples processing in 50 mL, 30 mL, and 7 mL tubes.
Figure 2. Protein yields from pea and spinach as a function of tube size: Normalized pea and spinach samples were homogenized on the Omni Bead Ruptor Elite in varying tube sizes and protein concentrations were determined.